Journal: bioRxiv
Article Title: Spatial growth in food-like matrices differentially modulates food-related stress responses but enhances digestive tolerance in major foodborne pathogens
doi: 10.64898/2026.03.05.710002
Figure Lengend Snippet: Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies isosurface after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Article Snippet: Raw fluorescence of each image was then binarised using the automated Imaris isosurface function.
Techniques: Incubation, Imaging, Staining, Confocal Laser Scanning Microscopy, Fluorescence, Software